Skip to main content
. 2021 Aug 11;13(1):151–171. doi: 10.1016/j.jcmgh.2021.08.002

Figure 1.

Figure 1

E-selectin is predominantly up-regulated in adipose tissue (AT) of patients and mice with NASH. (A) Normalized gene expression (microarray data sets obtained from Du Plessis et al9 and Lake et al25) in visceral adipose tissue and liver (L) of patients suffering from steatosis (nAT = 6, nL = 10) and NASH (nAT = 13, nL = 15) compared with those from healthy controls (nAT = 8, nL = 19). (B–E) WT mice were subjected to chow diet (Chow) (n = 7), a 3-month high-fat diet plus AdGfp (HFD) (n = 7) to induce steatosis or 3-month HFD plus AdCxcl1 to induce NASH (HFD+AdCxcl1) (n = 7). Adipose and liver tissues were collected and analyzed. (B) RT-qPCR of adipose tissue and liver. (C) Immunofluorescence staining of E-selectin (red), CD31 (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue) of adipose tissue. (D) MPO and F4/80 immunostaining of adipose tissues. (E) RT-qPCR of adipose tissue. ∗P < .05 for NASH vs normal, ∗∗P < .05 for steatosis vs normal or NASH vs normal. #P < .05.