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. 2021 Aug 11;13(1):151–171. doi: 10.1016/j.jcmgh.2021.08.002

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

E-selectin KO reduces hepatic fibrosis but not steatosis in HFD^+AdCxcl1-induced NASH. WT (n = 7) and Sele KO (n = 7) were subjected to 3-month HFD feeding plus AdCxcl1 to induce NASH. Liver tissues were collected and analyzed. (A) Sirius red staining of liver tissues. (B) RT-qPCR of liver tissues. (C) Western blot determination of α-smooth muscle actin (α-SMA) in liver tissues. In this panel, HFD feeding plus AdGfp (HFD) were used as controls. (D) Oil Red O staining of liver tissues. (E) Triglyceride (TG) determination of liver samples (n = 4). (F) RT-qPCR of liver tissues. ∗P < .05.