Table 1.
Antibodies
| Primary antibody | Dilution | Source |
|---|---|---|
| 4-HNE (mouse) | 1:400 (staining) 1:1000 (WB) | Genox, Baltimore, MD |
| ASK1 (rabbit) | 1:1000 | Thermo Fisher |
| p-ASK1 (Thr838) (rabbit) | 1:5000 | Thermo Fisher |
| CD31 (rabbit) | 1:200 | Cell Signaling Technologies |
| CLEC4F (rat) | 1:100 | R&D Systems |
| E-selectin (biotinylated) | 1:200 (staining) 1:5000 (WB) | R&D Systems |
| F4/80 (rabbit) | 1:400 | Cell Signaling Technology |
| IBA1 (rabbit) | 1:1000 | Avantor/VWR, Philadelphia, PA |
| JNK (rabbit) | 1:1000 | Cell Signaling Technology |
| p-JNK (Thr183/Tyr185) (rabbit) | 1:1000 | Cell Signaling Technology |
| MPO (rabbit) | 1:1 | Biocare Medical, Concord, CA |
| p38 (rabbit) | 1:1000 | Cell Signaling Technology |
| p-p38 (Thr180/Tyr182) (rabbit) | 1:1000 | Cell Signaling Technology |
| S100A8/A9 (mouse) | 1:500 (staining) 1:5000 (WB) | Novus Biologicals |
| α-SMA (mouse) | 1:5000 | Sigma-Aldrich |
| β-actin (mouse) | 1:5000 | Abcam, Cambridge, MA |
4-HNE, 4-hydroxynonenal; ASK1, apoptosis signal-regulating kinase 1; α-SMA, α-smooth muscle actin; CLEC4F, C-type lectin domain family 4 member F; IBA1, ionized calcium binding adaptor molecule 1; JNK, c-Jun-N-terminal kinase; p-ASK1, _phosphorylated ASK1; p-JNK, phosphorylated JNK; p-p38, phosphorylated p38 kinase; WB, Western blot.