Figure 1.

p70S6K staining is higher in cirrhotic vs healthy livers. (A) Representative images showing immunohistochemical staining of α-SMA and p70S6K in cirrhotic and healthy livers (brown staining and arrows). α-SMA staining is observed along the fibrous septa; p70S6K-positive cells are visible as clusters along or within the septa. (B) Immunohistochemical staining of α-SMA and p70S6K was quantified automatically from whole slide scans from the respective tissues samples. The data express the area with positive staining as a percentage of the total area (n = 5; ∗∗P < .01; t test). (C, D) A costaining of Hoechst (blue), p70S6K (green), and α-SMA or GFAP (both red) was performed in mice treated with vehicle (nonfibrotic control) or CCl₄. Control mice show a lower expression of p70S6K vs CCl₄-treated animals. The images show an overlapping (yellow) staining pattern of p70S6K (green) and α-SMA or GFAP (red). Scale bars = 100 μm.