Figure 5.

Spontaneous phenotype of p70S6K^–/– mice in comparison with Wt mice. (A, B) Comparison of age-matched Wt mice vs p70S6K^–/– mice did not show significant differences in body, liver, or spleen weight or in serum bilirubin, alanine aminotransferase (ALT), aspartate-aminotransferase (AST), and alkaline phosphatase (ALP). (C) Likewise, no differences were detected in the expression of α-SMA and collagen 1α1. (D, E) Hepatic fibrosis was assessed by Sirius red staining and quantitatively expressed as the fraction of Sirius red–positive area, which was quantified automatically from whole-slide scans. All figures are representative of experiments conducted from 3 independent samples showing no statistical differences.