Skip to main content
. 2021 Nov 16;12(6):e02975-21. doi: 10.1128/mBio.02975-21

FIG 3.

FIG 3

Binding breadth and neutralization of spike non-RBD mAbs. (a) Full-length spike protein binding to ACE2 (PDB accession number 7KJ2). (b to g) Locations of mutations found on B.1.1.7 (b), B.1.351 (c), P.1 (d), B.1.617.2 (e), B.1.526 (f), and B.1.617.1 (g) (modified from the structure under PDB accession number 6XM4). (h) Binding reactivity and neutralization capabilities 5 of NTA-A (pink)-, NTD-B (green)-, and S2 (purple)-reactive mAbs. The color gradients indicate the percentages of relative binding compared to WT spike. The neutralization potencies (IC50) of spike non-RBD mAbs against the WT and the B.1.1.7, P.1, B.1.617.2, and B.1.617.1 variants are indicated in nanograms per milliliter. The panel of SARS-CoV-2s is detailed in Table S4 in the supplemental material. Data in panel h are representative of results from two independent experiments performed in duplicate. Genetic information for each mAb can be found in Table S2.