TABLE 2.

Bacterial strains used, growth conditions selected, and known enzymes expressed under the conditions specified

Bacterial strain	Growth substrate/inducer	Relevant enzyme(s) expresseda	Reference(s)
Pseudomonas putida F1	l-Arginine	Toluene metabolism enzymes at very low levels	52
Pseudomonas putida F1	Toluene	Toluene pathway and regulatory proteins	52
Pseudomonas putida F39/D	Toluene	All toluene pathway enzymes with mutation in the diol dehydrogenase (TodD)	48
Escherichia coli K-12b	d-Glucose	NAd
Escherichia coli JM109 (pDTG601a)	IPTGc	Toluene dioxygenase	29
Escherichia coli JM109 (pDTG602)	IPTGc	Toluene dioxygenase and diol dehydrogenase	29
Pseudomonas sp. NCIB 9816	Naphthalene	Naphthalene dioxygenase pathway	53, 54
Pseudomonas sp. NCIB 9816-11	Naphthalene	Naphthalene enzymes with diol dehydrogenase mutation	53, 54
Burkholderia cepacia G4	Toluene	Toluene-2-monoxygenase pathway	55
Sphingobium yanoikuyae B1	Biphenyl	Biphenyl dioxygenase pathway	56
Paraburkholderia xenovorans LB400	Biphenyl	Biphenyl dioxygenase pathway	57

^aBased on peer-reviewed references.

^bNo aromatic ring oxygenases known to us induced with E. coli grown on glucose.

^cIPTG, isopropyl-β-d-1-thiogalactopyranoside; added to induce expression of the specified enzyme(s) indicated.

^dNA, not applicable.