FIG 4.
The conidiation-related genes brlA and flbC were required for asexual reproduction in the hapB mutant in submerged culture. (A and E) The transcript levels of the indicated genes in the ΔhapB, ΔhapB Tet-flbC, and ΔhapB Tet-brlA strains grown in liquid MM with or without 0.6 μM doxycycline (Dox) at 37°C for 48 h in a shaker culture. (B to D) A total of 3.5 × 106 spores were grown in liquid MM with or without 0.6 μM Dox at 37°C for 96 h. Asexual conidiogenous structure, macromorphology, and melanin of mycelial pellets were observed and measured. “(Off)” indicates the condition without Dox treatment. Scale bar represents 10 μm. Statistical significance was determined by Student’s t test. *, P < 0.05; **, P < 0.01; ns, not significant.