FIG 5.
Calcium enhanced the submerged conidiation of the CBC mutants. (A to C) A total of 3.5 × 106 spores were grown in liquid MM supplemented with indicated reagents in a shaker at 37°C for the indicated times, and the macromorphology, asexual conidiogenous structure, and melanin content of mycelial pellets were observed and measured. Scale bar represents 10 μm. (D) The relative transcript levels of the indicated genes between the ΔhapB and WT strains grown in liquid MM with/without 10 mM CaCl2 or 4 mM EGTA at 37°C for 48 h. Statistical significance was determined by Student’s t test. *, P < 0.05; **, P < 0.01.