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. 2021 Nov 2;12:774560. doi: 10.3389/fphar.2021.774560

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Copyright © 2021 Yue, Gao, Lv, Yu, Wang, Geng, Wang and Dou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Berberine inhibited intestinal GUS activity. (A) Fecal pellets were collected to evaluate the GUS activity in mice after CPT11 and CPT11 + berberine treatment by 4-MUG assay. (B) The assessment of fecal pellets for GUS-producing bacteria was determined by culture with 4-MUG agar medium. (C) Mice were treated with CPT11 or CPT11 + berberine for 14 days; GUS activity was then imaged using the fluorescent substrate FDGlcU. Data were expressed as the mean ± SD (n = 3 per group). ##p < 0.01, ###p < 0.001 vs. the control group; **p < 0.01, ***p < 0.001 vs. CPT1 + berberine treated group.