FIG 4.

EBNA1 attenuates cellular stress and apoptosis after EBV infection of B cells. (A) Representative immunofluorescence images of γH2AX foci (red) in uninfected primary B cells or B cells 4 days after infection with wt-EBV or ΔEBNA1-EBV. Green indicates GFP expression from either the recombinant wt-EBV or ΔEBNA1-EBV. (B) Fold enrichment of γH2AX focus-positive B cells infected with ΔEBNA1-EBV relative to cells infected with wt-EBV at 2, 4, and 6 days postinfection (n = 5; error bars show standard error). (C) Fold enrichment of Apologix-identified caspase-positive B cells infected with ΔEBNA1-EBV relative to cells infected with wt-EBV at 2, 4, and 6 days postinfection (n = 5; error bars show standard error). At least 50 cells were counted for each condition. *, P < 0.05.