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. 2021 Nov 16;12(6):e02188-21. doi: 10.1128/mBio.02188-21

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Copyright © 2021 Dmitriev et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — S. aureus srrAB alone responds to exogenous NO·. (A) Alignment of srrAB promoter regions from closely related staphylococcal species. Putative Rex (TGTGAW₆TCACA) and SrrA (AAATAN₄TTTAT) binding sights are outlined in red and green, respectively. (B) Expression of GFP driven by the S. aureus srrAB promoter responds to NO· (10 mM DETA-NO administered at OD₆₆₀ of 0.2), whereas srrAB promoters from other species do not. (C) Quantification of p_srrAB-GFP induction 30 min following NO· challenge from closely related staphylococcal species. Data were analyzed via one-way analysis of variance (ANOVA) with Dunnett’s correction for multiple comparisons (****, P ≤ 0.0001).