FIG 4.

S. aureus alone exhibits elevated respiratory capacity and NO· detoxification upon exposure to exogenous NO·. (A) NO· consumption rate by cells either prestimulated with NO· (10 mM DETA-NO for 1 h) or unstimulated. (B) Oxygen consumption rate before or after NO· exposure (10 mM DETA-NO for 1 h) using a Clark-type electrode. Oxygen consumption was initiated by addition of 0.01% glucose to washed and resuspended cells (OD₆₆₀, 1.0) (C) Concentration of NO· remaining in suspension when cells resume respiration. Cells were either prestimulated with NO· (10 mM DETA/NO for 1 h) or unstimulated. Statistical comparisons were carried out using a one-way ANOVA with Tukey’s posttest (****, P ≤ 0.0001; **, P ≤ 0.01; *, P ≤ 0.05).