TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus COL | Methicillin-resistant clinical isolate; laboratory strain | Laboratory strain |
| S. aureus LAC | Methicillin-resistant clinical isolate; laboratory strain | Laboratory strain |
| S. simiae | CCM 7213 | Laboratory strain |
| S. epidermidis | RP62A | Laboratory strain |
| S. saprophyticus | ATCC 15305 | Laboratory strain |
| S. haemolyticus | JCSC1435 | Laboratory strain |
| AR1593 | COL + pAD02 | This study |
| AR1612 | COL + pEP06 | This study |
| AR1606 | COL + pEP05 | This study |
| AR1600 | COL + pEP04 | This study |
| AR1569 | COL + pAD01 | This study |
| AR0352 | COL Δrex::Knr | 27 |
| AR1626 | COL ΔsrrB::Err (Φ11 NE588) | This study |
| AR1630 | COL Δrex::Knr, ΔsrrB::Err (Φ11 NE588) | This study |
| AR1315 | COL + pOS1-Plgt | This study |
| AR1408 | COL + pNV55 | This study |
| NE588 | SAUSA300_1441::Tn | 28 |
| Plasmids | ||
| pBT2ts | E. coli/S. aureus shuttle vector | 32 |
| pBTK | 1.4 kb aph-A3 allele cloned into SmaI of pBT2ts | 29 |
| pJF119 | CAT allele (Cmr) replacement of ApaI/XhoI Err region of promoterless GFP fusion vector pCN52 | 27, 30 |
| pAD01 | PsrrAB (S. haemolyticus) cloned into BamHI/EcoRI of pJF119 | This study |
| pAD02 | PsrrAB (S. aureus) cloned into BamHI/EcoRI of pJF119 | This study |
| pEP04 | PsrrAB (S. saprophyticus) cloned into BamHI/EcoRI of pJF119 | This study |
| pEP05 | PsrrAB (S. epidermidis) cloned into BamHI/EcoRI of pJF119 | This study |
| pEP06 | PsrrAB (S. simiae) cloned into BamHI/EcoRI of pJF119 | This study |
| pOS1-Plgt | S. aureus complementation vector driven by the lgt promoter | 31 |
| pNV55 | rex allele cloned into NdeI of pOS1-Plgt | This study |