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Binding properties of scDb/scFv-Fc and scDb/Fab-Fc variants. Binding to (A) LIM1215, (B) MCF-7, (C) SKBR-3, (D) FaDu, (E) HT1080, (F) MDA-MB-231 and (G) CD3-expressing Jurkat cells was analyzed in flow cytometry. A PE-labeled anti-huFc mAb was used to detect bound protein. Mean±SD, n=3. MFI, median fluorescence intensities; scDb, single-chain diabody.
