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. 2021 Nov 15;9(11):e003616. doi: 10.1136/jitc-2021-003616

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Activity of scDb/scFv-Fc or scDb/Fab-Fc variants on cytokine release, T-cell activation and proliferation. (A) IL-2 and (B) IFN-γ release mediated by scDb/scFv-Fc or scDb/Fab-Fc variants. Peripheral blood mononuclear cells were co-cultured with MCF-7 cells in the presence of a serial dilution of the scDb/scFv-Fc or scDb/Fab-Fc variants. Cytokine release was determined after 24 hours (IL-2) or 48 hours (IFN-γ) using sandwich ELISA. Proliferation of (C) CD4⁺ and (D) CD8⁺ T-cells was measured by carboxyfluorescein diacetate succinimidyl ester dilution in flow cytometry. Mean±SD, n=3. IFN-γ, interferon-γ; IL-2, interleukin 2; scDb, single-chain diabody.