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. 2021 Nov 15;9(11):e003616. doi: 10.1136/jitc-2021-003616

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Cytotoxic potential of peripheral blood mononuclear cells (PBMCs) stimulated with scDb/scFv-Fc or scDb/Fab-Fc variants. (A) LIM1215, (B) MCF-7, (C) SKBR-3, (D) FaDu, (E) HT1080 and (F) MDA-MB-231 cells were incubated with a serial dilution of scDb/scFv-Fc or scDb/Fab-Fc variants in the presence of PBMCs in an effector:target cell ratio (E:T) of 10:1. Cell viability was determined using crystal violet staining after 3 days. Mean±SD, n=3. scDb, single-chain diabody.