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Purification of rat liver mitochondria. (A) Outline of the mitochondrial fractionation scheme. Mitochondria from rat liver were isolated as described by Mihara and Omura (19) with subsequent purification by a linear Nycodenz gradient (10) in 0.22 M mannitol–0.075 M sucrose–10 mM HEPES-KOH (pH 7.4)–1 mM EDTA. (B) Electron micrograph of the purified mitochondria, demonstrating a high level of enrichment, estimated at >90% purity.
