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. 2021 Oct 28;23(1):11. doi: 10.3892/etm.2021.10933

Figure 4.

Figure 4

Effects of TF-3 on the protein expression levels of p38 MAPK, RIPK3, MLKL and caspase-3. BEAS-2B cells were treated with CSE (10%)/z-VAD (20 µM) alone or in combination with either TF-3 (5-20 µM) or SB203580 (0.5 µM). (A) Protein expression levels of p-p38 MAPK/p38 MAPK, p-RIPK3/RIPK3, p-MLKL/MLKL and caspase-3 were determined via western blotting with GAPDH as an internal control. Semi-quantification of the protein expression levels of (B) p-p38 MAPK, (C) p-RIPK3, (D) p-MLKL and (E) caspase-3. Data are presented as the mean ± SEM of three independent experiments indicated by dots. One-way ANOVA was performed followed by Tukey's post hoc test for statistical comparisons among more than two groups. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. CSE, cigarette smoke extract; TF-3, theaflavin-3,3'-digallate; RIPK3, receptor-interacting serine/threonine-protein kinase 3; MLKL, mixed lineage kinase domain-like; p, phosphorylated; cl, cleaved.