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. 2021 Oct 26;23(1):5. doi: 10.3892/etm.2021.10927

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Wnt5a overexpression blocks the inhibitory effect of irradiation on H1650 and A549 cell proliferation. (A) Overexpression of Wnt5a in transfected H1650 and A549 cells was confirmed by reverse-transcription quantitative PCR and immunoblotting, with GAPDH as a loading control. Proliferation of (B) H1650 and (C) A549 cells was determined by MTT assay following irradiation. Surviving fractions of (D and E) H1650 and (F and G) A549 cells were treated with a single dose of radiation (0-8 Gy) following transfection with Wnt5a or empty vector control (magnification, x10). Data are presented as the mean ± SD of three independent repeats. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. empty vector. Wnt5a, wingless-type protein 5a; si, small interfering.