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. 2021 Oct 28;23(1):12. doi: 10.3892/etm.2021.10934

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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NAT1 expression is decreased in MCF-7R cells and rescued by long non-coding RNA H19 knockdown. NAT1 (A) mRNA and (B) protein expression levels were detected using reverse transcription-quantitative PCR and western blot analysis, respectively, in various cell lines. Representative blots are shown with lanes as follows: lane 1, MCF-7; lane 2, MCF-7R, lane 3, MCF-7R/shH19; lane 4, MCF-7/NC; lane 5, MCF-7R/siH19; lane 6, MCF-7R/NS siRNA. All data are presented as the mean ± SEM of three independent experiments. ^*P<0.05 and ^***P<0.05 as indicated. NAT1, N-acetyltransferase 1; MCF-7R, tamoxifen-resistant MCF-7 cells; siH19, siRNA targeting H19; NS, non-specific; siRNA, small interfering RNA; shH19, shRNA targeting H19; NC, negative control shRNA; shRNA, short hairpin RNA.