Table 1.

Overview of the targets and their respective DNA templates used in multiplex qPCR assay screenings

Target	Canine blood	Tick	Flea	Limit of detection (copies/PCR)	References
Babesia rossi	X	X		5	[24]
Babesia canis	X	X		5	[24]
Ehrlichia canis	X	X		5	[25]
Ehrlichia chaffeensis	X	X		5	[26]
Anaplasma platys	X	X		16	[27]
Anaplasma phagocytophilum	X	X		9	[28]
Rickettsia conorii	X	X		8	[29]
Rickettsia africae	X	X		8	[29]
Coxiella burnetti	X	X		8	[30]
Hepatozoon canis	X	X		5	In-houseb
Dirofilaria immitis	X			8	[31]
Trypanosoma spp.a	X			5	[32, 33]
Bartonella henselae			X	5	[34]
Mycoplasma haemofelis			X	16	[35]
Babesia felis		X		8	In-houseb
Dipylidium caninum			X	8	[36]

^aviz. Trypanosoma vivax, T. congolense, T. evansi and T. brucei

^bHydrolysis probe was designed in-house (Clinomics, Bloemfontein, South Africa)