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. 2021 Nov 16;9:222. doi: 10.1186/s40168-021-01160-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Polar plots showing peak relative abundance of significantly rhythmic microbiome MetaCycle pathways. Each circle represents a pathway, coloured by MetaCycle class and sized by average relative abundance. Pathway radian indicates time of peak and distance from centre indicates significance (more significant/stronger rhythms towards edge of plot). Pathway identity determined via Picrust2 and rhythmicity significance determined via eJTK_cycle (Bonferoni-corrected P values < 0.05). Circacompare was used to fit waveforms and determine estimates of rhythms peaks. A, B, C = healthy trout under 12:12 LD. D, E, F = Argulus-infected trout under 12:12 LD. H, I, J = healthy trout under 24:0 LD. K, L, M = Argulus-infected trout under 24:0 LD. Full details of pathways are provided in Supplementary Datafile 1