Fig. 6.

Effects of PJH on CS/LPS-induced changes in NF-κB and ERK1/2 activation. Western blot analysis was performed in lung tissues to examine the phosphorylation of NF-κB and ERK1/2. CON: control group; CS + LPS group: CS/LPS-exposed; RO group: administration of roflumilast 10 mg/kg prior to CS/LPS exposure; PJH100 group: administration of PJH 100 mg/kg prior to CS/LPS exposure; PJH200 group: administration of PJH 200 mg/kg prior to CS/LPS exposure. Results are presented as means ± SD (^#P < 0.05, ^##P < 0.01 compared with the CON group; *P < 0.05, **P < 0.01 compared with the CS + LPS group). Gels/blots are cropped for a clear presentation of results. Full-length blots/gels are presented in Supplementary Fig. 1. Protein samples derived from the same experiment and gels/blots were processed in parallel