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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: J Am Chem Soc. 2021 Aug 30;143(36):14676–14683. doi: 10.1021/jacs.1c05908

Figure 2.

Figure 2.

Characterization of Cel-cest-1.2-dependent metabolites. (a) Abundances of glucoside scaffolds in Cel-cest-1.2 mutants relative to wildtype C. elegans. (b) ESI− ion chromatograms for 2-O-acylated iglu#121 (25) and its 6-O-acylated isomer, iglu#12 (15), in Cel-cest-1.2 and wildtype C. elegans, showing abolishment specifically of the 2-O-acylated isomer. (c) Abundances of 2-O- (red) vs 6-O- (green) monoacylated MOGLs in Cel-cest-1.2 and Cbr-cest-2 mutants relative to wildtype C. elegans (N2) or wildtype C. briggsae (AF16), respectively. Data and error bars represent the mean of 4 biological replicates and standard deviation. (d) Synthetic scheme of 2-O-acylated MOGLs iglu#101 (26), iglu#121 (25), and iglu#401 (28) from iglu#1 (4).14,21