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. Author manuscript; available in PMC: 2021 Nov 16.
Published in final edited form as: FASEB J. 2021 Apr;35(4):e21402. doi: 10.1096/fj.202000938RR

FIGURE 4.

FIGURE 4

MVC loading and release in synthesized PSMA-b-PS NPs. A, One-step RAFT polymerizations were used to synthesize amphiphilic diblock copolymers using maleic anhydride (MA) and styrene (STY) monomers. In the presence of water, the diblock copolymers self-assemble into micelle NPs with a hydrophilic corona and a hydrophobic core. B, PSMA-b-PS NPs are functionalized with TRAP-binding peptide (TBP) via carbodiimide chemistry and loaded with MVC to produce TBP-NPMVC. C, Release of free MVC and MVC from TBP-NPs in 0.1 mol/L PBS pH 7.4, 37°C show sustained release from TBP-NPs. Data represents mean ± std. (n = 3). Statistical differences were tested via a paired two-way ANOVA followed by Sidak’s multiple comparisons test for free MVC and TBP-NPMVC at each time point. *P < .05, ****P < .0001, ns = not significant