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Pseudouridylation. (A) The pseudouridylation reaction results in the conversion of uridine to pseudouridine by rotating the molecule in 180° across its N3–C6 axis (represented by the diagonal line). (B) Cartoon representation displaying the stacked helices and three-way junctions (J1 and J2) formed by the interaction between guide H/ACA RNA and substrate RNA. The target uridine is marked in the figure by an arrow. (PDB: 2P89 [Wu and Feigon 2007].)
