Fig. 2.

BoMoC acts as a terminal transferase in the presence of Mn²⁺. SYBR Gold-stained denaturing PAGE gels of the terminal transferase reaction products of BoMoC are shown. (A) Input single-stranded (ss) RNA and DNA and double-stranded (ds) blunt-ended RNA, DNA, and RNA-DNA duplex were assayed in Mg²⁺ and Mn²⁺ reaction conditions in the presence of 500 μM of each dNTP or dATP alone. Products marked with asterisks indicate template copying primed by a noncomplementary oligonucleotide. (B) RNA oligonucleotide with 3′C or 3′U was assayed for extension by a single ddNTP in Mg²⁺ and Mn²⁺ conditions in the presence of 500 μM of an individual ddNTP. Single NTA in Mg²⁺ results from cDNA synthesis priming by a noncomplementary oligonucleotide. (C) RNA oligonucleotide with 3′G or 3′A was assayed for extension under Mn²⁺ conditions in the presence of 500 μM of an individual rNTP.