TABLE 1.
Mutations in the HindIII-HpaI region
| Mutation | Wild-type sequence | Mutant sequence | New restriction site |
|---|---|---|---|
| M1 | TTTCCTATAGTT | GGGATCCGCTGG | BamHI |
| M2 | GAAAAGCTCA | AGGCCTAGAG | StuI |
| M3 | AGAGAATTGA | CTCTCCGGAC | BspEI |
| M4 | GTATAAGTTT | TGCGCCCGGG | SmaI |
| M5 | ATGAGTGCTT | CGTCCCATGG | NcoI |
| M6 | AGCGGCAAAC | CTATTGGCCA | MscI |
| M7 | GCACCATCAG | TACGTCGACT | SalI |
| M8 | AGTTTTTTCC | CCGGGGGGAA | SmaI |
| M9 | TTCATGGAGC | GCACGTTCTA | FspI |
| M10 | GACAGTTTGC | TCACTGGGAA | EcoRI |
| M11 | GATTTGCCCG | ACGCGTAAAT | MluI |
| M12 | AGCGTGAAAG | CTATGTTCTA | XbaI |
| M13 | AAGCTTCCCG | CCATGGAAAT | NcoI |