Table 2. IspC Apparent Kinetic Parameters.
| AbIspC | KpIspC | EcIspCa | FtIspCa | MtIspCa | YpIspCa | |
|---|---|---|---|---|---|---|
| KMapp (DXP) (μM)b | 127.6 ± 7.2 | 155.5 ± 10.8 | 81–250 | 103.7 ± 12.1 | 47 | 221.5 ± 34.3 |
| KMapp(NADPH)(μM) | 19.53 ± 0.53 | 14.51 ± 0.83 | 0.5–18 | 13.3 ± 1.5 | 29.7 | 12.7 ± 1.5 |
| kcatDXP (s–1)b | 1.92 ± 0.03 | 2.21 ± 0.06 | 33 | 2.0 ± 0.09 | 1.2 | 1.7 |
| kcatNADPH (s–1) | 1.64 ± 0.01 | 1.96 ± 0.03 | 1.3 ± 0.04 | 1.0 | ||
| kcatDXP/KMDXP (M–1 min–1) | 9.0 × 105 ± 5.3 × 104 | 8.5 × 105 ± 6.3 × 104 | 2.4 × 107 | 1.2 × 106 | 1.5 × 106 | 4.6 × 105 |
| IC50fosmidomycin (nM)c | 46.8 (38.2–57.2) | 20.2 (15.9–25.6) | 35 | 247 | 80 | 710 |
| IC50FR900098 (nM) | 23.9 (21.4–26.7) | 23.1 (18.3–29.2) | 35 | 230 | 160 | 231 |
| ref | This study | This study | (40,74−76) | (35,72) | (71,77) | (57) |
a
EcIspC, FtIspC, MtIspC, YpIspC = recombinant IspC from Escherichia coli, Francisella tularensis, Mycobacterium tuberculosis, and Yersinia pestis, respectively.
b
KMapp and kcat values were obtained using GraphPad Prism 5.0.
c
The 95% confidence intervals of the IC50 values are indicated parenthetically. All assays were performed in duplicate. The standard errors for the KMapp and kcat values for DXP and NADPH, and kcatDXP/KMDXP are indicated for AbIspC and KpIspC.