C. burnetii intracellular replication potential is dependent on host cell type and nutritional environment. C. burnetii intracellular replication was measured during infection of J774A.1 (A), JEG-3 (B), THP-1 (C), and VERO (D) cells cultured in complete RPMI or IFmM. Replication was determined via quantification of GE every 2 days for 8 days. Depicted data illustrate means ± SEM (n = 3 to 6). *, P < 0.05; **, P < 0.01 (unpaired Student's t test).