FIG 2.

Medium glucose and amino acid levels directly influence C. burnetii intracellular replication in a cell type-dependent manner. To assess the influence of specific nutrients provided to the host on C. burnetii intracellular replication, J774A.1 (A), VERO (B), and JEG-3 (C) cells were infected with C. burnetii expressing GFP and cultured in modified IFmM with increasing amounts of glucose or altered concentrations of amino acids (AAs). C. burnetii yields were determined via enumeration of GE at 5 days p.i., and values presented as percent of control (complete IFmM, 100%, dashed line). Depicted data illustrate means ± SEM (n = 3 to 8). *, P < 0.05; ****, P < 0.0001 (one-way analysis of variance [ANOVA] with Dunnett’s posttest applied to panels A and C). (D) Representative fluorescence micrographs of GFP expressing C. burnetii 5 days p.i. under the conditions described for panels A to C.