TABLE 1.
Primers used for amplification or qRT-PCR
| Gene | Forward primer (5′—3′) | Reverse primer (5′—3′) |
|---|---|---|
| Sod2 (human)b | TGTAATCAACTGGGAGAA | CTTAGAAGACAGGACATTATC |
| Sod2 (human)a (for pcDNA3.1 plasmid) | ACCACGATCGTTATGCTGATCATACCC | GATGGTTGACAGATTCTTTTATTAACA |
| Sod2 (human)a (for pmirGLO plasmid) | GAGCTCACCACGATCGTTATGCTGATCATACCC | GTCGACGATGGTTGACAGATTCTTTTATTAACA |
| β-actin (human)b | TGACGTGGACATCCGCAAAG | CTGGAAGGTGGACAGCGAGG |
| U6b | GTAATACGACTCACTATAGGGAGAAGAG | CGCGCCTGCAGGTCGAC |
| miR-495b | GGAAACAAACATGGTGCACTTCTT | Provided in kit |
| Sod2 (mouse)b | AGAATGTTACTGAAAGATA | ATGCTCTACACTACTATA |
| β-actin (mouse)b | CTTCCTTCTTGGGTATGG | CACTGTGTTGGCATAGAG |
a
Used for amplification.
b
Used for qRT-PCR.