FIG. 2.

Increases in RLI and RNase L proteins during the differentiation of transfected C2 cells. (A) Pooled C2 cells stably transfected with an RLI cDNA sense construct (VS) and an RLI cDNA antisense construction (VAS) were grown in GM and then shifted to DM on day 1. At the indicated times, cells were lysed, and RLI expression was assessed in protein samples (100 μg) using an RLI-specific antiserum. (B) Densitometric analysis of the gel shown in panel A. A value of 100% corresponds to the amount of RLI protein at day 0 in proliferating C2 control myoblasts. Error bars refer to the standard deviation obtained in three independent experiments. Symbols: ◊, VS cells; ■, VAS cells. (C) The cells described in panel A were lysed, and proteins (600 μg) were incubated with radiolabeled 2-5A₄-3′-[³²P]pCp (2-5ApCp) in a radiobinding assay; 100% corresponds to the amount of 2-5ApCp bound to RNase L in proliferating C2 control myoblasts. Error bars refer to the standard deviation obtained in three independent experiments. Symbols: ◊, VS cells; ■, VAS cells.