FIG. 3.

RLI mRNA levels increase during the differentiation of transfected C2 cells. Pooled stable control C2 cells transfected by an empty pcDNA3 vector (VV) (A), pooled stable C2 cells transfected with an RLI cDNA antisense construction (VAS) (B), and pooled stable C2 cells transfected with an RLI cDNA sense construction (VS) (C) were grown in GM and then shifted to DM on day 1. At the indicated times, cells were harvested, and mRNAs were extracted and analyzed by Northern blot using the murine RLI [³²P]cDNA probe (VV, VAS, and VS [□]). The VS samples were also probed with a human RLI [³²P]cDNA fragment (■) as indicated. Densitometric analysis of the gels are presented. A value of 100% corresponds to the amount of RLI mRNA in proliferating C2 control myoblasts at day 0. Error bars refer to standard deviation obtained in three independent experiments.