TABLE 1.

Expression levels and putative functions of pneumococcal genes that are sensitive to temperature changes in wild-type D39 at mid-exponential growth phase^a

Locus	Function(s)	Fold change at:
		34°C	40°C
SPD_0035-SPD_0036	Hypothetical protein	2.32 to 3	−1.93 to −2.18
SPD_0046-SPD_0047	Bacteriocin synthesis	9.33 to 9.35	−7.69 to −7.89
SPD_0049-SPD_0056	Competence development, purine metabolism	2.58 to 14.81	−1.95 to −9.74
SPD_0059-SPD_0060	Purine metabolism	1.92 to 2.79
SPD_0093-SPD_0096	Hypothetical protein	−2.54 to −3.02
SPD_0113-SPD_0116	Hypothetical protein	−2.9 to −4.01	19.9 to 32.51
SPD_0132-SPD_0133	Hypothetical protein	6.23 to 52.94	−5.8 to −31.81
SPD_0452-SPD_0453	Phage integrase protein, restriction/modification subunit	−2.35 to −2.37
SPD_0447-SPD_0448	MerR family protein, glutamine synthetase	−3.66 to −4.66	2.96 to 4.12
SPD_0466-SPD_0468	Bacteriocin synthesis	2.49 to 7.01	−2.41 to −6.49
SPD_0470-SPD_0474	Bacteriocin synthesis	3.38 to 16.54	−2.02 to −9.59
SPD_0701-SPD_0702	Histidine kinase, response regulator	1.96 to 2.06	−2.24 to −2.59
SPD_0723-SPD_0724	Sugar metabolism		−1.99 to −1.9
SPD_0771-SPD_0773	Sugar metabolism	1.91 to 2.12	−2.46 to −3.04
SPD_1098-SPD_1099	ABC transporter	−2.9 to −3.44	2.68 to 2.79
SPD_1175-SPD_1179	Hypothetical protein, ABC transporter protein		1.97 to 2.48
SPD_1380-SPD_1381	Hypothetical protein, unclassified metabolism	1.98 to 2.35
SPD_1514-SPD_1516	ABC transporter protein, hypothetical protein		2.74 to 3.99
SPD_1527-SPD_1528	Hypothetical protein, ABC transporter protein	2.06 to 2.9	−2.02 to −2.29
SPD_1650-SPD_1652	ABC transporter protein	2.61 to 4.01
SPD_1682-SPD_1698	tRNA synthesis	−2.01 to −2.83	2.15 to 3.41
SPD_1740-SPD_1744	Competence damage protein, hypothetical protein	3.1 to 5.84	−3.22 to −5.37
SPD_1830-SPD_1831	Sugar metabolism, PTS transporter system	1.97 to 2.21
SPD_1855-SPD_1856	Hypothetical protein	3.71 to 6.18	−2.42 to −5.38
SPD_1857-SPD_1862	Competence development	7.65 to 37.39	−5.64 to −20.39
SPD_2033-SPD_2035	Ribosomal surface protein, competence protein, helicase	2.82 to 3.55
SPD_2063-SPD_2065	Competence development	35.18 to 53.65	−23.95 to −44.92
SPD_2068-SPD_2069	Serine protease, transcriptional regulator SpoJ	4.4 to 6.38	−3.5 to −3.73

^aFold changes are approximately ≥2.0 or less than or equal to −2.0 of each operon. All P values are <0.001.