TABLE 2.
Cell size of wild-type D39, ΔgdhA, and complemented mutant strain, CompΔgdhA, grown in CDM supplemented with 55 mM glucose at 34°C, 37°C, and 40°Ca
Strain | Cell size (μm) at: |
||
---|---|---|---|
34°C | 37°C | 40°C | |
D39 (WT) | 0.44 ± 0.01 | 0.48 ± 0.01 | 0.36 ± 0.00 |
ΔgdhA | 0.39 ± 0.00 | 0.46 ± 0.00 | 0.25 ± 0.01 |
CompΔgdhA | 0.45 ± 0.03 | 0.48 ± 0.06 | 0.34 ± 0.03 |
Cell size of D39 wild type reduced when grown at 34°C or 40°C compared to 37°C (P < 0.05). A similar size trend was recorded in the absence of gdhA (P < 0.05). While no difference was observed between D39 and ΔgdhA at 37°C, ΔgdhA cells were significantly smaller than the wild type at 34°C or 40°C. No difference between the wild type and complemented mutant strain was seen. The standard error shows the mean of three different experiments for at least 50 individual cell measurements.