TABLE 3.

Glutamate dehydrogenase activity in the wild type (WT), ΔgdhA, and CompΔgdhA at 34°C, 37°C, and 40°C

Strain	Glutamate dehydrogenase activity (mU/mg protein) ata:
	34°C	37°C	40°C
WT	16.51 ± 0.65	38.19 ± 0.66	74.48 ± 0.83
ΔgdhA	1.54 ± 0.35	2.51 ± 0.19	0.04 ± 0.02
CompΔgdhA	13.25 ± 0.79	28.89 ± 0.51	57.59 ± 2.27

^aThe enzyme activity is expressed as nmol/ml (mU/ml) of NADH released from the substrate per minute at pH 7.6 and normalized against mg protein and expressed for 1 × 10⁸ CFU. The highest induction of GdhA was obtained at 40°C in the wild type and complemented mutant strain CompΔgdhA. The activity in ΔgdhA was negligible at all temperatures.