FIG 1.

PacBio sequencing reveals structural differences between the genomes of Acinetobacter baumanii 17978 VU and 17978 UN variants. The genome sequences of A. baumanii 17978 VU and 17978 UN variants were determined by PacBio and Nanopore sequencing and subsequently compared to the genome of the reference strain, A. baumannii ATCC 17978-mff (RefSeq accession number NZ_CP012004.1). (A) Visual representation of AbaAL44, encompassing multiple accessory genes present in the genome of A. baumannii 17978 UN, based on analysis and inspection of a new A. baumannii 17978 UN reference genome (NCBI accession number CP079931). The relative position (in terms of base pair number) within the assembled genome of A. baumannii 17978 UN is indicated above each row, and gene names are indicated below each gene. Locus tags refer to CP079931. Arrows indicate the relative transcriptional direction of each gene. Genes indicated in red represent putative pathogenesis genes. (B) Images of agarose gel electrophoresis indicating the differential presence of the accessory clsC2 gene within individual colonies of A. baumannii 17978 laboratory stocks based on PCR detection of clsC2. The VUMC laboratory stock (left) was obtained from ATCC in 2009, and the UIC laboratory stock (right) was obtained from ATCC in 2021. Presence of accessory clsC2 indicates that an isolate belongs to A. baumannii 17978 UN variant.