FIG 5.

AbaAL44 clsC2 and katX mutants generated in the A. baumannii 17978 UN background exhibit growth defects in vitro. (A and B) Stationary-phase cultures of the indicated strains of A. baumannii were normalized to a similar optical density at 600 nm (OD₆₀₀) and inoculated into fresh rich medium (LB) or minimal medium (Tris minimal succinate [TMS]). Growth was assayed by measuring OD₆₀₀ over time. Symbols depict the mean, and error bars show standard deviation of the mean. N = 3 or 4 biological replicates per group per experiment. Graphs depict representative data from two independent experiments. For 8-, 10-, and 12-h time points, the means of A. baumannii 17978 UN ΔclsC2, 17978 UN ΔclsC2/clsC2, 17978 UN ΔkatX/EV, 17978 UN ΔkatX/pkatX, and 17978 UN variants were compared using a one-way ANOVA adjusted for multiple comparisons. (A) Differences between A. baumannii 17978 UN and 17978 UN ΔclsC2, 17978 UN ΔclsC2/clsC2, or 17978 UN ΔkatX/EV variants: P < 0.0001 for each comparison for each time point. Difference between A. baumannii 17978 UN and 17978 UN ΔkatX/pkatX variants: P < 0.05 for the10-h time point and P > 0.05 for 8- and 12-h time points. (B) Differences between A. baumannii 17978 UN and 17978 UN ΔclsC2, 17978 UN ΔclsC2/clsC2, or 17978 UN ΔkatX/pkatX variants: P > 0.05 for each comparison for each time point. Difference between A. baumannii 17978 UN and 17978 UN ΔkatX/EV variants: P < 0.0001, P < 0.0001, or P < 0.001 for 8-, 10-, and 12-h time points, respectively.