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Single-plane time-lapse video of an explant generated from an embryo expressing Jupiter::GFP (green) and H2Av::RFP (magenta), after addition of Nocodazole on the left side of the explant using a fine micropipette, aiming for a final concentration of ∼4 µM. Time in min:s; scale bar, 50 µm; acquired at 2 frames min−1; and displayed at 5 frames s−1.
