Figure 2.

Quaking viable mice (qk^v) display decreased macrophage influx and decreased interstitial fibrosis upon unilateral urethral obstruction (UUO). (A) mRNA expression of QKI mRNA levels in cultured macrophages of either qk^v or wild-type littermate controls. (B) mRNA levels of macrophage markers (F4/80, CD115) in whole kidney lysates derived from either qk^v mice (gray bars) or wild-type littermate controls on day 5 and 10 after UUO. (C) Western blot analysis of whole UUO kidney lysates for α-SMA, CD206 and histone H3. Quantitation using ImageJ software is provided below. (D) mRNA levels of fibrosis markers (α-SMA and COL1A1) in whole kidney lysates derived from either qk^v mice (gray bars) or wild-type littermate controls (open bars) on day 5 and 10 after UUO. (E) Representative photomicrographs of Sirius Red staining for collagen on 4 μm sections of 5- or 10-day UUO kidneys from either qk^v or wild-type littermate controls. (F) Quantitation of Sirius red staining is provided. (G) Pearson R correlation is plotted for COL1A1 and F4/80 expression. * p ≤ 0.05 by Students’ t-test, error bars represent the SEM.