Table 1.

Description of the amplicons. The table shows the NCBI accession numbers, exons covered by nested PCR products and nested product lengths created using the primer sets described previously [23].

Gene	NCBI IDs	Analysed Exons	Amplicon Length (bp) **	mCpGs/ Amplicon	Comments
Dynactin * dctn4 subunit 4	XP_006560650	4, 5, 6	551	10	Differentially spliced, methylated in queen and worker adult brain and in larval head
Nadrin, rho GTPase-activating protein	XP_006572269	10	405	15	Differentially spliced, methylated in queen and worker brain
PKCbp1, protein kinase C-binding protein	XP_016769618	5, 6	401	10	Methylated gene, different isoforms expressed in adult brains and larval heads

* The BeeBase annotation of Dynactin shows a gene model with 10 exons, whereas the NCBI shows a gene model with 9 exons. The extra exon in BeeBase is located between NCBI exons 7 and 8. Our RNA seq data support the NCBI model (see Figure S2 for dctn4 gene model and References [16] and [24] for accession numbers). ** Not including adaptors and indices.