Figure 1.

Construction of epiRILs (hypomethylated population) for quantitative epigenetics. In the left side, the scheme for the construction of hypomethylated population using demethylating agent 5-AzaCytidine is shown. On the right side, scheme for construction of epiRILs with stable inheritance by crossing of two parents (wild-type and epimutator parents (met1 or ddm1)) with different epigenetic states is shown. The green and red circles that overlay the genome sequence illustrates the different epigenetic states of the two parents.