Table 1.

Summarized results of PCM and the direct “on chip quantification” demonstrating the FS 100% bacteria capture efficiency.

	Biol Rep 1			Biol Rep 2			Biol Rep 3
	Tech Rep 1 [Ave cfu/mL]	Tech Rep 2 [Ave cfu/mL]	Tech Rep 3 [Ave cfu/mL]	Tech Rep 1 [Ave cfu/mL]	Tech Rep 2 [Ave cfu/mL]	Tech Rep 3 [Ave cfu/mL]	Tech Rep 1 [Ave cfu/mL]	Tech Rep 2 [Ave cfu/mL]	Tech Rep 3 [Ave cfu/mL]
Plate-count method [cfu/mL] “PCM quantification”
NegCTRL (FS control buffer)	0	0	0	0	0	0	0	0	0
Influent ≤ 250 cfu/mL	70	20	17	52	12	42	65	53	20
Effluent	0	0	0	0	0	0	0	0	0
Capeff [%]	100	100	100	100	100	100	100	100	100
Direct FS baseline of particles “on-chip quantification” [particles/mL]
FS baseline of particles	6	15	3	4	3	12	26	13	5
Direct total particle capture “on-chip quantification” [particles/mL]
Total particle capture	184	130	198	124	179	189	124	128	139
Direct “on-chip quantification” (Total particle capture on-chip minus FS baseline of particles on-chip) [bacteria/mL]
Total capture	178	115	195	120	176	177	98	115	134

Cap_eff, Capture efficiency. The flow rate used in the Fluid-Screen bacterial capture and separation experiments is 300 µL/min.