Figure 2.

Effects of 0.72 mg E₂ on bone mineral density and structure in tumor-naive young vs. skeletally mature mice. (A) Tibial areal bone mineral density (BMD) in young (4-week-old) vs. skeletally mature (15-week-old) mice supplemented with 0.72 mg E₂ (vs. control), as measured by DXA (n = 6–8/group). Arrows indicate time of E₂ pellet placement. *P ≤ 0.01, **P ≤ 0.0001 vs. age-matched control, by 2-way ANOVA with Sidak post-test. (B) Representative microCT images of tibial cortical (top) and trabecular (bottom) bone of young vs. skeletally mature E₂ (0.72 mg)-supplemented mice (vs. age-matched controls), 6 weeks after E₂ pellet placement.