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. 2021 Oct 13;10:e70934. doi: 10.7554/eLife.70934

Figure 2. CLE40 and CLV3 show complementary expression patterns in the inflorescence meristem (IFM).

(A) Maximum intensity projection (MIP) of an inflorescence at 5 weeks after germination (WAG) expressing the transcriptional reporter CLE40:Venus-H2B//Col-0 showing CLE40 expression in the IFM, older primordia and sepals (N = 23). (B) The L1 projection shows high expression in the epidermis of the periphery of the IFM and only weak expression in the central zone (CZ). (C) Longitudinal section through the IFM shows expression of CLE40 in the periphery, but downregulated expression in the CZ. (P1 –P6) Longitudinal section through primordia show no CLE40 expression in young primordia (P1–P4), but in the centre of older primordia (P5–P6). (D) The MIP of the double reporter line of CLE40 and CLV3 (CLE40:Venus-H2B;CLV3:NLS-3xmCherry//Col-0) shows CLV3 expression in the CZ surrounded by CLE40 expression in the periphery (N = 12). (E–E’’’) The L1 projection shows CLV3 (E’) expression in the centre of the IFM and CLE40 (E’’) expression in a distinct complementary pattern in the periphery of the IFM. (F) The longitudinal section through the centre of the IFM shows CLV3 expression in the CZ while CLE40 (F’) is mostly expressed in the surrounding cells. (F’’) CLE40 and CLV3 are expressed in complementary patterns. Dashed blue lines indicate magnified areas, dashed white and orange lines indicate planes of longitudinal sections, dashed red line in (A) and (D) marks the IFM area, the dashed pink line marks the sepals, the dashed rose line marks the FMs and dashed yellow line in (F’’) the OC. Scale bars: 50 µm (A, D), 20 µm (B, C, E, E’’’, F’’), 10 µm (P0–P6), PI: propidium iodide; L1: visualization of layer 1 only; P1–P7: primordia at consecutive stages.

Figure 2.

Figure 2—figure supplement 1. CLE40 transcriptional and translational reporter lines display same expression patterns.

Figure 2—figure supplement 1.

(A) The CLE40:Venus-H2B reporter line shows expression in the columella cells (CC) of the distal root meristem (DRM) and in the stele of main root at 5 days after germination (DAG). (B) The translational CLE40:CLE40-GFP reporter shows also expression of CLE40 in the stele and CCs of the DRM of lateral roots. (C) Roots of cle40-2 mutants carrying the translational CLE40:CLE40-GFP//cle40-2 and cle40-2 mutants seedlings at 11 DAG. (D) Root length of cle40-2 mutants in comparison to Col-0 and cle40-2 mutants carrying a CLE40:CLE40-GFP construct at 11 DAG. The CLE40:CLE40-GFP transgene restores wild-type root growth in cle40-2 mutants. Stars indicate significant differences after calculating p-values by ANOVA and Dunnett’s multiple comparison test (differential grouping from p≤0.0001), N ≥ 79.
Figure 2—figure supplement 2. CLE40 and CLV3 expression in multiple inflorescence meristem (IFM).

Figure 2—figure supplement 2.

(A–E) Maximum intensity projections (MIPs) of four independent T1 lines of IFMs at 5 weeks after germination (WAG) expressing the transcriptional reporter CLE40:Venus-H2B//Col-0 showing CLE40 expression in the IFM, flower meristem (FMs) and sepals. (A’–E’) XZ sections through the IFM of (A–E), respectively. XZ sections show expression of CLE40 in the periphery, but downregulated expression in the central zone (CZ) and organizing centre (OC). (F–J) MIP of four different IFMs at 5 WAG expressing the transcriptional reporter CLE40:Venus-H2B;CLV3:NLS-3xmCherry//Col-0 showing CLE40 expression in the IFM, FMs and sepals and CLV3 expression in the CZ. (F’–J’) XZ sections through the IFM of (F–J), respectively. XZ sections show expression of CLE40 in the periphery, but downregulated expression in the CZ and OC. CLV3 is expressed in the stem cells in the CZ in a cone-shaped pattern. Dashed orange line indicates the planes of longitudinal sections; Scale bars: 50 µm (A–J), 20 µm (A’–J’).