|
bam1-3
|
PCR |
bam1-3_F: ctaacgactctccgggagctbam1-3_R: taaggaccacagagatcaggattacLbaI_R: tggttcacgtagtgggccatcg |
WT amp.: 1208 bpmutant amp.: 998 bp |
|
cle40-2
|
dCAPS |
cle40-2_F: GGAGAAACACAAGATACGAAAGCCATGcle40-2_R: ATTGTGATTTGATACCAACTTAAAA |
Restriction enzyme: AseIWT amp.: 460 + 200 bpmutant amp.: 410 + 200 + 60 bp |
|
cle40-cr1
|
dCAPS |
cle40-cr_F: ATGGCGGCGATGAAATACAAcle40-cr_R: GTTACGCTTTGGCATCTTTCC |
Restriction enzyme: BamHIWT amp.: 750 bpmutant amp.: 491 + 259 bp |
|
cle40-cr2
|
|
cle40-cr3
|
|
clv1-20
|
PCR |
clv1-20_F: TTTGAATAGTGTGTGACCAAATTTGAclv1-20_R: TCCAATGGTAATTCACCGGTGLBa.1: TGGTTCACGTAGTGGGCCATCG |
WT amp.: 860 bpmutant amp: 1200 bp |
|
clv1-101
|
PCR |
clv1-101_F: TTCTCCAAATTCACCAACAGGclv1-101_R: CAACGGAGAAATCCCTAAAGGWiscLox_LT6_R: AATAGCCTTTACTTGAGTTGGCGTAAAAG |
WT amp.: 1158 bpmutant amp.: 896 bp |
|
wus-7
|
dCAPS |
wus-7_F: CCGACCAAGAAAGCGGCAACAwus-7_R: AGACGTTCTTGCCCTGAATCTTT |
Restriction enzyme: XmnIWT amplification: 216 bpmutant amp.: 193 + 23 bp |
