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| Fluorophore/staining | Excitation (nm) | Emission (nm) | MBS | Detector | Light source |
|---|---|---|---|---|---|
| DAPI | 405 | 410–490 | 405 | PMT | Diode |
| GFP | 488 | 500–545 | 488/561 | GaAsP | Argon laser |
| Venus | 514 | 518–540 | 458/514 | GaAsP | Argon laser |
| mCherry | 561 | 570–640 | 458/561 | PMT | DPSS laser |
| PI | 561 | 595–650 | 488/561 | PMT | DPSS laser |
PMT, photomultiplier tubes; DPSS, diode-pumped solid state.
