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. 2021 Oct 22;10:e67292. doi: 10.7554/eLife.67292

Figure 5. Carbamylcholine (CCh)-induced goblet cell-associated antigen passage (GAP) formation and mucus secretion use different muscarinic acetylcholine (ACh) receptors.

Effect of the muscarinic ACh receptor 4 (mAChR4) antagonist tropicamide (Trop.), the mAChR1 antagonist telenzepine (Telenz.), or the preferential mAChR3 antagonist 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (4-DAMP) on CCh-induced mucus secretion and GAP formation in the small intestine (SI) villus (A–B), the SI crypt (C–D), and the distal colon crypt (E–F). Data are presented as mean ± SEM. **p < 0.01, ***p < 0.001, n.s = non-significant, as compared to CCh. A–B, D–E, n = 6 in all groups. (C and F) Vehicle and CCh n = 7, CCh + 4 DAMP and CCh + Telenz. n = 5. Statistical analysis was performed using a one-way ANOVA followed by Dunnet’s post hoc test. Each data point represents the average of 25 villi or 40 crypts from one mouse.

Figure 5.

Figure 5—figure supplement 1. Small intestine expression of muscarinic ACh receptor 4 (mAChR4) and mAchR1.

Figure 5—figure supplement 1.

Confocal fluorescent imaging of (A) mAChR4 and (B) mAChR1 expression in the small intestine using RNAscope. Arrows denote goblet cells staining positive for the mAChR1/4 probes. Scale bar 50 µm. n = 5.
Figure 5—figure supplement 2. Distal colon expression of muscarinic ACh receptor 3 (mAChR3) and mAChR1.

Figure 5—figure supplement 2.

Confocal fluorescent imaging of (A) mAChR3 and (B) mAChR1 expression in the distal colon using RNAscope. Arrows denote goblet cells staining positive for the mAChR1/3 probes. Scale bar 50 µm. n = 5.